Anti-Phospho-MEK5 (Ser311 Thr315) Antibody

Product Name

Anti-Phospho-MEK5 (Ser311 Thr315) Antibody

View all MEK5 Antibodies

SKU/Catalog Number

P03980

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-MEK5 (Ser311 Thr315) Antibody (Catalog # P03980). Tested in WB applications. This antibody reacts with Rat.

Storage & Handling

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Cite This Product

Anti-Phospho-MEK5 (Ser311 Thr315) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P03980)

Host

Rabbit

Contents

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Clonality

Polyclonal

Clone Number

1B9

Isotype

IgG

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser311/Thr315 of rat MEK5, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Rat.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

P03980 is reactive to MAP2K5 in Rat

Applications

P03980 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

52 kDa

Calculated molecular weight

50.112kDa

Background of MEK5

MEK5 (also known as MKK5) is a dual specificity serine/threonine protein kinase belonging to the MAP kinase kinase family. MEK5 has been shown to specifically activate ERK5 (Zhou et al., 1995) whereas MEK5 itself is regulated by MEKK3 (Chao et al., 1999). An important link between MEK5 and metastatic prostrate cancer has been demonstrated (Mehta et.al., 2003). Dual phosphorylation of Ser-311 and Thr-315 have been implicated in cell proliferation (Cameron et al., 2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000

Validation Images & Assay Conditions

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Western blot of rat testes lysate showing specific labeling of the ~49 kDa MEK5 protein phosphorylated at Ser³¹¹ Thr³¹⁵ in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

Gene/Protein Information For MAP2K5 (Source: Uniprot.org, NCBI)

Alternative Names

HsT17454; MAP kinase kinase 5; MAP kinase kinase MEK5b; MAP2K5; MAPK/ERK kinase 5; MAPKK 5; MAPKK5; MAPKK5EC 2.7.12.2; MEK5; MEK5MEK 5; mitogen-activated protein kinase kinase 5; MKK5; PRKMK5; PRKMK5dual specificity mitogen-activated protein kinase kinase 5 MAP2K5 HsT17454, MAPKK5, MEK5, PRKMK5 mitogen-activated protein kinase kinase 5 dual specificity mitogen-activated protein kinase kinase 5|MAP kinase kinase 5|MAP kinase kinase MEK5b|MAPK/ERK kinase 5|MAPKK 5|MEK 5

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